Construct: shRNA TRCN0000375147

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018888.3-1491s21c1
DNA Barcode:
TGTGCTTGGAGCAGTTTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Uqcc1 (56046)
Gene Description:
ubiquinol-cytochrome c reductase complex assembly factor 1
Transcript:
RefSeq NM_018888.3 (NON-CURRENT)
Match location:
Position 1491 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000375147)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTGCTTGGAGCAGTTTATTA
Hairpin Sequence:
5'-CCGG-TGTGCTTGGAGCAGTTTATTA-CTCGAG-TAATAAACTGCTCCAAGCACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTGCTTGGAGCAGTTTATTACTCGAGTAATAAACTGCTCCAAGCACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTGCTTGGAGCAGTTTATTACTCGAGTAATAAACTGCTCCAAGCACA-3'

Other clones with same target sequence:

(none)