Construct: shRNA TRCN0000375153

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025369.2-274s21c1
DNA Barcode:
ACCAGACACTGCAGAAATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mrps36 (66128)
Gene Description:
mitochondrial ribosomal protein S36
Transcript:
RefSeq NM_025369.2 (NON-CURRENT)
Match location:
Position 274 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000375153)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCAGACACTGCAGAAATTAT
Hairpin Sequence:
5'-CCGG-ACCAGACACTGCAGAAATTAT-CTCGAG-ATAATTTCTGCAGTGTCTGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCAGACACTGCAGAAATTATCTCGAGATAATTTCTGCAGTGTCTGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCAGACACTGCAGAAATTATCTCGAGATAATTTCTGCAGTGTCTGGT-3'

Other clones with same target sequence:

(none)