Construct: shRNA TRCN0000375161

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026202.3-721s21c1
DNA Barcode:
GGAAATTGCTCGACTTCTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccdc50 (67501)
Gene Description:
coiled-coil domain containing 50
Transcript:
RefSeq NM_026202.3 (NON-CURRENT)
Match location:
Position 721 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000375161)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAAATTGCTCGACTTCTAAT
Hairpin Sequence:
5'-CCGG-GGAAATTGCTCGACTTCTAAT-CTCGAG-ATTAGAAGTCGAGCAATTTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAAATTGCTCGACTTCTAATCTCGAGATTAGAAGTCGAGCAATTTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAAATTGCTCGACTTCTAATCTCGAGATTAGAAGTCGAGCAATTTCC-3'

Other clones with same target sequence:

(none)