Construct: shRNA TRCN0000375194

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011916.2-5334s21c1
DNA Barcode:
TGATATCTATGTGTCAGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Xrn1 (24127)
Gene Description:
5'-3' exoribonuclease 1
Transcript:
RefSeq NM_011916.2 (NON-CURRENT)
Match location:
Position 5334 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000375194)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATATCTATGTGTCAGAAAT
Hairpin Sequence:
5'-CCGG-TGATATCTATGTGTCAGAAAT-CTCGAG-ATTTCTGACACATAGATATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATATCTATGTGTCAGAAATCTCGAGATTTCTGACACATAGATATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATATCTATGTGTCAGAAATCTCGAGATTTCTGACACATAGATATCA-3'

Other clones with same target sequence:

(none)