Construct: shRNA TRCN0000375203

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133215.1-3929s21c1
DNA Barcode:
AGGTCCCAAGATGATTCATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mtmr4 (170749)
Gene Description:
myotubularin related protein 4
Transcript:
RefSeq NM_133215.1 (CURRENT)
Match location:
Position 3929 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000375203)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGTCCCAAGATGATTCATAA
Hairpin Sequence:
5'-CCGG-AGGTCCCAAGATGATTCATAA-CTCGAG-TTATGAATCATCTTGGGACCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGTCCCAAGATGATTCATAACTCGAGTTATGAATCATCTTGGGACCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGTCCCAAGATGATTCATAACTCGAGTTATGAATCATCTTGGGACCT-3'

Other clones with same target sequence:

(none)