Construct: shRNA TRCN0000375242

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007598.3-608s21c1
DNA Barcode:
CAGAGCTTACTTGAGTATATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cap1 (12331)
Gene Description:
CAP, adenylate cyclase-associated protein 1 (yeast)
Transcript:
RefSeq NM_007598.3 (NON-CURRENT)
Match location:
Position 608 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000375242)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAGCTTACTTGAGTATATG
Hairpin Sequence:
5'-CCGG-CAGAGCTTACTTGAGTATATG-CTCGAG-CATATACTCAAGTAAGCTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAGCTTACTTGAGTATATGCTCGAGCATATACTCAAGTAAGCTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAGCTTACTTGAGTATATGCTCGAGCATATACTCAAGTAAGCTCTG-3'

Other clones with same target sequence:

(none)