Construct: shRNA TRCN0000375314

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011603.5-1310s21c1
DNA Barcode:
GGGCACCAAAGAACCTGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tbpl1 (237336)
Gene Description:
TATA box binding protein-like 1
Transcript:
RefSeq NM_011603.5 (CURRENT)
Match location:
Position 1310 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000375314)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGCACCAAAGAACCTGTAAA
Hairpin Sequence:
5'-CCGG-GGGCACCAAAGAACCTGTAAA-CTCGAG-TTTACAGGTTCTTTGGTGCCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGCACCAAAGAACCTGTAAACTCGAGTTTACAGGTTCTTTGGTGCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGCACCAAAGAACCTGTAAACTCGAGTTTACAGGTTCTTTGGTGCCC-3'

Other clones with same target sequence:

(none)