Construct: shRNA TRCN0000375393

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183138.2-2681s21c1
DNA Barcode:
CTGATACCCTCCGGAAGTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tet3 (194388)
Gene Description:
tet methylcytosine dioxygenase 3
Transcript:
RefSeq NM_183138.2 (CURRENT)
Match location:
Position 2681 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000375393)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGATACCCTCCGGAAGTATG
Hairpin Sequence:
5'-CCGG-CTGATACCCTCCGGAAGTATG-CTCGAG-CATACTTCCGGAGGGTATCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGATACCCTCCGGAAGTATGCTCGAGCATACTTCCGGAGGGTATCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGATACCCTCCGGAAGTATGCTCGAGCATACTTCCGGAGGGTATCAG-3'

Other clones with same target sequence:

(none)