Construct: shRNA TRCN0000375548

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026143.2-1879s21c1
DNA Barcode:
TAAATTGGAGCGTGGAGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Far1 (67420)
Gene Description:
fatty acyl CoA reductase 1
Transcript:
RefSeq NM_026143.2 (NON-CURRENT)
Match location:
Position 1879 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000375548)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAAATTGGAGCGTGGAGTAAA
Hairpin Sequence:
5'-CCGG-TAAATTGGAGCGTGGAGTAAA-CTCGAG-TTTACTCCACGCTCCAATTTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAAATTGGAGCGTGGAGTAAACTCGAGTTTACTCCACGCTCCAATTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAAATTGGAGCGTGGAGTAAACTCGAGTTTACTCCACGCTCCAATTTA-3'

Other clones with same target sequence:

(none)