Construct: shRNA TRCN0000375710

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029277.2-3111s21c1
DNA Barcode:
AGTCATATACACTGGATAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arhgap12 (75415)
Gene Description:
Rho GTPase activating protein 12
Transcript:
RefSeq NM_029277.2 (CURRENT)
Match location:
Position 3111 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000375710)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTCATATACACTGGATAATT
Hairpin Sequence:
5'-CCGG-AGTCATATACACTGGATAATT-CTCGAG-AATTATCCAGTGTATATGACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTCATATACACTGGATAATTCTCGAGAATTATCCAGTGTATATGACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTCATATACACTGGATAATTCTCGAGAATTATCCAGTGTATATGACT-3'

Other clones with same target sequence:

(none)