Construct: shRNA TRCN0000375723

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009108.2-873s21c1
DNA Barcode:
GCCTCAGGAAATCACAAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nr1h4 (20186)
Gene Description:
nuclear receptor subfamily 1, group H, member 4
Transcript:
RefSeq NM_009108.2 (CURRENT)
Match location:
Position 873 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000375723)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCAGGAAATCACAAATAA
Hairpin Sequence:
5'-CCGG-GCCTCAGGAAATCACAAATAA-CTCGAG-TTATTTGTGATTTCCTGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCAGGAAATCACAAATAACTCGAGTTATTTGTGATTTCCTGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCAGGAAATCACAAATAACTCGAGTTATTTGTGATTTCCTGAGGC-3'

Other clones with same target sequence:

(none)