Construct: shRNA TRCN0000375737

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023061.2-1045s21c1
DNA Barcode:
TGGAGCTGCTGGTGAACTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mcam (84004)
Gene Description:
melanoma cell adhesion molecule
Transcript:
RefSeq NM_023061.2 (CURRENT)
Match location:
Position 1045 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000375737)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGAGCTGCTGGTGAACTATG
Hairpin Sequence:
5'-CCGG-TGGAGCTGCTGGTGAACTATG-CTCGAG-CATAGTTCACCAGCAGCTCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGAGCTGCTGGTGAACTATGCTCGAGCATAGTTCACCAGCAGCTCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGAGCTGCTGGTGAACTATGCTCGAGCATAGTTCACCAGCAGCTCCA-3'

Other clones with same target sequence:

(none)