Construct: shRNA TRCN0000375821

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022019.5-780s21c1
DNA Barcode:
CACTGTCTTAGACTTGATTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dusp10 (63953)
Gene Description:
dual specificity phosphatase 10
Transcript:
RefSeq NM_022019.5 (CURRENT)
Match location:
Position 780 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000375821)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTGTCTTAGACTTGATTTC
Hairpin Sequence:
5'-CCGG-CACTGTCTTAGACTTGATTTC-CTCGAG-GAAATCAAGTCTAAGACAGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTGTCTTAGACTTGATTTCCTCGAGGAAATCAAGTCTAAGACAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTGTCTTAGACTTGATTTCCTCGAGGAAATCAAGTCTAAGACAGTG-3'

Other clones with same target sequence:

(none)