Construct: shRNA TRCN0000375977

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053168.1-1385s21c1
DNA Barcode:
GATGGGTCGCTGCTGTTTATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trim11 (94091)
Gene Description:
tripartite motif-containing 11
Transcript:
RefSeq NM_053168.1 (NON-CURRENT)
Match location:
Position 1385 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000375977)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGGGTCGCTGCTGTTTATC
Hairpin Sequence:
5'-CCGG-GATGGGTCGCTGCTGTTTATC-CTCGAG-GATAAACAGCAGCGACCCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGGGTCGCTGCTGTTTATCCTCGAGGATAAACAGCAGCGACCCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGGGTCGCTGCTGTTTATCCTCGAGGATAAACAGCAGCGACCCATC-3'

Other clones with same target sequence:

(none)