Construct: shRNA TRCN0000375990

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023854.1-1945s21c1
DNA Barcode:
TGCAGGCTCTAGGATCCTTGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arfgap2 (77038)
Gene Description:
ADP-ribosylation factor GTPase activating protein 2
Transcript:
RefSeq NM_023854.1 (NON-CURRENT)
Match location:
Position 1945 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000375990)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCAGGCTCTAGGATCCTTGT
Hairpin Sequence:
5'-CCGG-TGCAGGCTCTAGGATCCTTGT-CTCGAG-ACAAGGATCCTAGAGCCTGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCAGGCTCTAGGATCCTTGTCTCGAGACAAGGATCCTAGAGCCTGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCAGGCTCTAGGATCCTTGTCTCGAGACAAGGATCCTAGAGCCTGCA-3'

Other clones with same target sequence:

(none)