Construct: shRNA TRCN0000376006

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007604.2-745s21c1
DNA Barcode:
ACTGCCATCAGTGAGAATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Capza2 (12343)
Gene Description:
capping protein (actin filament) muscle Z-line, alpha 2
Transcript:
RefSeq NM_007604.2 (NON-CURRENT)
Match location:
Position 745 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000376006)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGCCATCAGTGAGAATTAT
Hairpin Sequence:
5'-CCGG-ACTGCCATCAGTGAGAATTAT-CTCGAG-ATAATTCTCACTGATGGCAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGCCATCAGTGAGAATTATCTCGAGATAATTCTCACTGATGGCAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGCCATCAGTGAGAATTATCTCGAGATAATTCTCACTGATGGCAGT-3'

Other clones with same target sequence:

(none)