Construct: shRNA TRCN0000376026

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177762.6-2946s21c1
DNA Barcode:
CTCTCTGCTCCCACTCATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aak1 (269774)
Gene Description:
AP2 associated kinase 1
Transcript:
RefSeq NM_177762.6 (CURRENT)
Match location:
Position 2946 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000376026)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTCTGCTCCCACTCATAAA
Hairpin Sequence:
5'-CCGG-CTCTCTGCTCCCACTCATAAA-CTCGAG-TTTATGAGTGGGAGCAGAGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTCTGCTCCCACTCATAAACTCGAGTTTATGAGTGGGAGCAGAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTCTGCTCCCACTCATAAACTCGAGTTTATGAGTGGGAGCAGAGAG-3'

Other clones with same target sequence:

(none)