Construct: shRNA TRCN0000376136

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175510.3-5597s21c1
DNA Barcode:
GTACGTCGCACAGGAATTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Unc80 (329178)
Gene Description:
unc-80, NALCN activator
Transcript:
RefSeq NM_175510.3 (CURRENT)
Match location:
Position 5597 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000376136)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACGTCGCACAGGAATTATT
Hairpin Sequence:
5'-CCGG-GTACGTCGCACAGGAATTATT-CTCGAG-AATAATTCCTGTGCGACGTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACGTCGCACAGGAATTATTCTCGAGAATAATTCCTGTGCGACGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACGTCGCACAGGAATTATTCTCGAGAATAATTCCTGTGCGACGTAC-3'

Other clones with same target sequence:

(none)