Construct: shRNA TRCN0000376144

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001013012.1-617s21c1
DNA Barcode:
ACAGAGCAAGAGCCTAGCCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp787 (67109)
Gene Description:
zinc finger protein 787
Transcript:
RefSeq NM_001013012.1 (CURRENT)
Match location:
Position 617 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000376144)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGAGCAAGAGCCTAGCCAA
Hairpin Sequence:
5'-CCGG-ACAGAGCAAGAGCCTAGCCAA-CTCGAG-TTGGCTAGGCTCTTGCTCTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGAGCAAGAGCCTAGCCAACTCGAGTTGGCTAGGCTCTTGCTCTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGAGCAAGAGCCTAGCCAACTCGAGTTGGCTAGGCTCTTGCTCTGT-3'

Other clones with same target sequence:

(none)