Construct: shRNA TRCN0000376172

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_194345.1-980s21c1
DNA Barcode:
CCAGCAGACATTGCCACTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam160b2 (239170)
Gene Description:
family with sequence similarity 160, member B2
Transcript:
RefSeq NM_194345.1 (CURRENT)
Match location:
Position 980 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000376172)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCAGACATTGCCACTTTA
Hairpin Sequence:
5'-CCGG-CCAGCAGACATTGCCACTTTA-CTCGAG-TAAAGTGGCAATGTCTGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCAGACATTGCCACTTTACTCGAGTAAAGTGGCAATGTCTGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCAGACATTGCCACTTTACTCGAGTAAAGTGGCAATGTCTGCTGG-3'

Other clones with same target sequence:

(none)