Construct: shRNA TRCN0000376193

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001164669.1-592s21c1
DNA Barcode:
GACCGAGAAGAAGTCATTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dnah6 (330355)
Gene Description:
dynein, axonemal, heavy chain 6
Transcript:
RefSeq NM_001164669.1 (CURRENT)
Match location:
Position 592 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000376193)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCGAGAAGAAGTCATTAAA
Hairpin Sequence:
5'-CCGG-GACCGAGAAGAAGTCATTAAA-CTCGAG-TTTAATGACTTCTTCTCGGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCGAGAAGAAGTCATTAAACTCGAGTTTAATGACTTCTTCTCGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCGAGAAGAAGTCATTAAACTCGAGTTTAATGACTTCTTCTCGGTC-3'

Other clones with same target sequence:

(none)