Construct: shRNA TRCN0000376231

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001164493.1-1700s21c1
DNA Barcode:
ACGCAGAGCGAAAGCATTTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Klhl29 (208439)
Gene Description:
kelch-like 29
Transcript:
RefSeq NM_001164493.1 (CURRENT)
Match location:
Position 1700 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000376231)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGCAGAGCGAAAGCATTTAC
Hairpin Sequence:
5'-CCGG-ACGCAGAGCGAAAGCATTTAC-CTCGAG-GTAAATGCTTTCGCTCTGCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGCAGAGCGAAAGCATTTACCTCGAGGTAAATGCTTTCGCTCTGCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGCAGAGCGAAAGCATTTACCTCGAGGTAAATGCTTTCGCTCTGCGT-3'

Other clones with same target sequence:

(none)