Construct: shRNA TRCN0000376272

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001164285.1-1141s21c1
DNA Barcode:
TTATATGCTTTGGATCTTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
C87414 (381654)
Gene Description:
expressed sequence C87414
Transcript:
RefSeq NM_001164285.1 (CURRENT)
Match location:
Position 1141 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000376272)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTATATGCTTTGGATCTTATG
Hairpin Sequence:
5'-CCGG-TTATATGCTTTGGATCTTATG-CTCGAG-CATAAGATCCAAAGCATATAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTATATGCTTTGGATCTTATGCTCGAGCATAAGATCCAAAGCATATAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTATATGCTTTGGATCTTATGCTCGAGCATAAGATCCAAAGCATATAA-3'

Other clones with same target sequence:

(none)