Construct: shRNA TRCN0000376386

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031881.2-951s21c1
DNA Barcode:
GACAATTTAGGCCGAACTTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nedd4l (83814)
Gene Description:
neural precursor cell expressed, developmentally down-regulated gene 4-like
Transcript:
RefSeq NM_031881.2 (CURRENT)
Match location:
Position 951 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000376386)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACAATTTAGGCCGAACTTAC
Hairpin Sequence:
5'-CCGG-GACAATTTAGGCCGAACTTAC-CTCGAG-GTAAGTTCGGCCTAAATTGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACAATTTAGGCCGAACTTACCTCGAGGTAAGTTCGGCCTAAATTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACAATTTAGGCCGAACTTACCTCGAGGTAAGTTCGGCCTAAATTGTC-3'

Other clones with same target sequence:

(none)