Construct: shRNA TRCN0000376539

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012319.3-1752s21c1
DNA Barcode:
GATCGAACTGAAGGCTATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC39A6 (25800)
Gene Description:
solute carrier family 39 member 6
Transcript:
RefSeq NM_012319.3 (NON-CURRENT)
Match location:
Position 1752 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000376539)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCGAACTGAAGGCTATTTA
Hairpin Sequence:
5'-CCGG-GATCGAACTGAAGGCTATTTA-CTCGAG-TAAATAGCCTTCAGTTCGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCGAACTGAAGGCTATTTACTCGAGTAAATAGCCTTCAGTTCGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCGAACTGAAGGCTATTTACTCGAGTAAATAGCCTTCAGTTCGATC-3'

Other clones with same target sequence:

(none)