Construct: shRNA TRCN0000376754

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010435.2-3935s21c1
DNA Barcode:
TGGGTGGACATGGACTATATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hira (15260)
Gene Description:
histone cell cycle regulator
Transcript:
RefSeq NM_010435.2 (CURRENT)
Match location:
Position 3935 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000376754)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGGTGGACATGGACTATATC
Hairpin Sequence:
5'-CCGG-TGGGTGGACATGGACTATATC-CTCGAG-GATATAGTCCATGTCCACCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGGTGGACATGGACTATATCCTCGAGGATATAGTCCATGTCCACCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGGTGGACATGGACTATATCCTCGAGGATATAGTCCATGTCCACCCA-3'

Other clones with same target sequence:

(none)