Construct: shRNA TRCN0000376796

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016857.2-128s21c1
DNA Barcode:
GGAGGAGACGCTGTCGTTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Exoc7 (53413)
Gene Description:
exocyst complex component 7
Transcript:
RefSeq NM_016857.2 (CURRENT)
Match location:
Position 128 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000376796)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAGGAGACGCTGTCGTTTAT
Hairpin Sequence:
5'-CCGG-GGAGGAGACGCTGTCGTTTAT-CTCGAG-ATAAACGACAGCGTCTCCTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAGGAGACGCTGTCGTTTATCTCGAGATAAACGACAGCGTCTCCTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAGGAGACGCTGTCGTTTATCTCGAGATAAACGACAGCGTCTCCTCC-3'

Other clones with same target sequence:

(none)