Construct: shRNA TRCN0000376920

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009713.3-2378s21c1
DNA Barcode:
TGCTGTTCGGAATGGGAAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arsa (11883)
Gene Description:
arylsulfatase A
Transcript:
RefSeq NM_009713.3 (NON-CURRENT)
Match location:
Position 2378 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000376920)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTGTTCGGAATGGGAAATA
Hairpin Sequence:
5'-CCGG-TGCTGTTCGGAATGGGAAATA-CTCGAG-TATTTCCCATTCCGAACAGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTGTTCGGAATGGGAAATACTCGAGTATTTCCCATTCCGAACAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTGTTCGGAATGGGAAATACTCGAGTATTTCCCATTCCGAACAGCA-3'

Other clones with same target sequence:

(none)