Construct: shRNA TRCN0000376940

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026959.2-1149s21c1
DNA Barcode:
ACGTGCCAGCATGTGTCTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stx18 (71116)
Gene Description:
syntaxin 18
Transcript:
RefSeq NM_026959.2 (NON-CURRENT)
Match location:
Position 1149 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000376940)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGTGCCAGCATGTGTCTCAT
Hairpin Sequence:
5'-CCGG-ACGTGCCAGCATGTGTCTCAT-CTCGAG-ATGAGACACATGCTGGCACGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGTGCCAGCATGTGTCTCATCTCGAGATGAGACACATGCTGGCACGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGTGCCAGCATGTGTCTCATCTCGAGATGAGACACATGCTGGCACGT-3'

Other clones with same target sequence:

(none)