Construct: shRNA TRCN0000377041

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033324.2-2647s21c1
DNA Barcode:
CTTGTGCACAGTCGATGTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dgcr8 (94223)
Gene Description:
DGCR8, microprocessor complex subunit
Transcript:
RefSeq NM_033324.2 (CURRENT)
Match location:
Position 2647 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000377041)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGTGCACAGTCGATGTATG
Hairpin Sequence:
5'-CCGG-CTTGTGCACAGTCGATGTATG-CTCGAG-CATACATCGACTGTGCACAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGTGCACAGTCGATGTATGCTCGAGCATACATCGACTGTGCACAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGTGCACAGTCGATGTATGCTCGAGCATACATCGACTGTGCACAAG-3'

Other clones with same target sequence:

(none)