Construct: shRNA TRCN0000377049

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133697.3-223s21c1
DNA Barcode:
AGTCCTACTGCACCGACACAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smim14 (68552)
Gene Description:
small integral membrane protein 14
Transcript:
RefSeq NM_133697.3 (CURRENT)
Match location:
Position 223 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000377049)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTCCTACTGCACCGACACAG
Hairpin Sequence:
5'-CCGG-AGTCCTACTGCACCGACACAG-CTCGAG-CTGTGTCGGTGCAGTAGGACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTCCTACTGCACCGACACAGCTCGAGCTGTGTCGGTGCAGTAGGACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTCCTACTGCACCGACACAGCTCGAGCTGTGTCGGTGCAGTAGGACT-3'

Other clones with same target sequence:

(none)