Construct: shRNA TRCN0000377060

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007729.2-6096s21c1
DNA Barcode:
ATCCATCAAAGATAGAGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Col11a1 (12814)
Gene Description:
collagen, type XI, alpha 1
Transcript:
RefSeq NM_007729.2 (NON-CURRENT)
Match location:
Position 6096 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000377060)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCCATCAAAGATAGAGTAAA
Hairpin Sequence:
5'-CCGG-ATCCATCAAAGATAGAGTAAA-CTCGAG-TTTACTCTATCTTTGATGGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCCATCAAAGATAGAGTAAACTCGAGTTTACTCTATCTTTGATGGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCCATCAAAGATAGAGTAAACTCGAGTTTACTCTATCTTTGATGGAT-3'

Other clones with same target sequence:

(none)