Construct: shRNA TRCN0000377063

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026446.3-860s21c1
DNA Barcode:
TGGCAACTGCAGGGTTCTAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rgs19 (56470)
Gene Description:
regulator of G-protein signaling 19
Transcript:
RefSeq NM_026446.3 (NON-CURRENT)
Match location:
Position 860 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000377063)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGCAACTGCAGGGTTCTAAG
Hairpin Sequence:
5'-CCGG-TGGCAACTGCAGGGTTCTAAG-CTCGAG-CTTAGAACCCTGCAGTTGCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGCAACTGCAGGGTTCTAAGCTCGAGCTTAGAACCCTGCAGTTGCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGCAACTGCAGGGTTCTAAGCTCGAGCTTAGAACCCTGCAGTTGCCA-3'

Other clones with same target sequence:

(none)