Construct: shRNA TRCN0000377106

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019734.2-532s21c1
DNA Barcode:
CCATGTGTACATCAATCATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Asah1 (11886)
Gene Description:
N-acylsphingosine amidohydrolase 1
Transcript:
RefSeq NM_019734.2 (NON-CURRENT)
Match location:
Position 532 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000377106)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGTGTACATCAATCATAA
Hairpin Sequence:
5'-CCGG-CCATGTGTACATCAATCATAA-CTCGAG-TTATGATTGATGTACACATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGTGTACATCAATCATAACTCGAGTTATGATTGATGTACACATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGTGTACATCAATCATAACTCGAGTTATGATTGATGTACACATGG-3'

Other clones with same target sequence:

(none)