Construct: shRNA TRCN0000377305

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006395.2-1249s21c1
DNA Barcode:
GGCGTGAGACACATCACATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATG7 (10533)
Gene Description:
autophagy related 7
Transcript:
RefSeq NM_006395.2 (NON-CURRENT)
Match location:
Position 1249 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000377305)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCGTGAGACACATCACATTT
Hairpin Sequence:
5'-CCGG-GGCGTGAGACACATCACATTT-CTCGAG-AAATGTGATGTGTCTCACGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCGTGAGACACATCACATTTCTCGAGAAATGTGATGTGTCTCACGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCGTGAGACACATCACATTTCTCGAGAAATGTGATGTGTCTCACGCC-3'

Other clones with same target sequence:

(none)