Construct: shRNA TRCN0000377328

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024490.3-1448s21c1
DNA Barcode:
GAATATTCTCATGATGCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP10A (57194)
Gene Description:
ATPase phospholipid transporting 10A (putative)
Transcript:
RefSeq NM_024490.3 (CURRENT)
Match location:
Position 1448 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000377328)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATATTCTCATGATGCAAAT
Hairpin Sequence:
5'-CCGG-GAATATTCTCATGATGCAAAT-CTCGAG-ATTTGCATCATGAGAATATTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATATTCTCATGATGCAAATCTCGAGATTTGCATCATGAGAATATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATATTCTCATGATGCAAATCTCGAGATTTGCATCATGAGAATATTC-3'

Other clones with same target sequence:

(none)