Construct: shRNA TRCN0000377372

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004477.2-631s21c1
DNA Barcode:
TGGCTTTGTTGGCCTCAAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FRG1 (2483)
Gene Description:
FSHD region gene 1
Transcript:
RefSeq NM_004477.2 (NON-CURRENT)
Match location:
Position 631 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000377372)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGCTTTGTTGGCCTCAAATA
Hairpin Sequence:
5'-CCGG-TGGCTTTGTTGGCCTCAAATA-CTCGAG-TATTTGAGGCCAACAAAGCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGCTTTGTTGGCCTCAAATACTCGAGTATTTGAGGCCAACAAAGCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGCTTTGTTGGCCTCAAATACTCGAGTATTTGAGGCCAACAAAGCCA-3'

Other clones with same target sequence:

(none)