Construct: shRNA TRCN0000377378

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004393.2-3597s21c1
DNA Barcode:
TTACGTTTAATCTGCTGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DAG1 (1605)
Gene Description:
dystroglycan 1
Transcript:
RefSeq NM_004393.2 (NON-CURRENT)
Match location:
Position 3597 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000377378)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTACGTTTAATCTGCTGTTTA
Hairpin Sequence:
5'-CCGG-TTACGTTTAATCTGCTGTTTA-CTCGAG-TAAACAGCAGATTAAACGTAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTACGTTTAATCTGCTGTTTACTCGAGTAAACAGCAGATTAAACGTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTACGTTTAATCTGCTGTTTACTCGAGTAAACAGCAGATTAAACGTAA-3'

Other clones with same target sequence:

(none)