Construct: shRNA TRCN0000377528

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024104.3-97s21c1
DNA Barcode:
CGGTGCTGAACTTTAAGCTGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMIM7 (79086)
Gene Description:
small integral membrane protein 7
Transcript:
RefSeq NM_024104.3 (CURRENT)
Match location:
Position 97 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000377528)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTGCTGAACTTTAAGCTGA
Hairpin Sequence:
5'-CCGG-CGGTGCTGAACTTTAAGCTGA-CTCGAG-TCAGCTTAAAGTTCAGCACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTGCTGAACTTTAAGCTGACTCGAGTCAGCTTAAAGTTCAGCACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTGCTGAACTTTAAGCTGACTCGAGTCAGCTTAAAGTTCAGCACCG-3'

Other clones with same target sequence:

(none)