Construct: shRNA TRCN0000377614

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198402.3-1008s21c1
DNA Barcode:
ACACCCTGGCCAGTTCATTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HACD2 (201562)
Gene Description:
3-hydroxyacyl-CoA dehydratase 2
Transcript:
RefSeq NM_198402.3 (NON-CURRENT)
Match location:
Position 1008 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000377614)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACACCCTGGCCAGTTCATTTC
Hairpin Sequence:
5'-CCGG-ACACCCTGGCCAGTTCATTTC-CTCGAG-GAAATGAACTGGCCAGGGTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACACCCTGGCCAGTTCATTTCCTCGAGGAAATGAACTGGCCAGGGTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACACCCTGGCCAGTTCATTTCCTCGAGGAAATGAACTGGCCAGGGTGT-3'

Other clones with same target sequence:

(none)