Construct: shRNA TRCN0000377650

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001145194.1-385s21c1
DNA Barcode:
AGTTAACCAAGAAGCACGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AKAIN1 (642597)
Gene Description:
A-kinase anchor inhibitor 1
Transcript:
RefSeq NM_001145194.1 (NON-CURRENT)
Match location:
Position 385 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000377650)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTTAACCAAGAAGCACGAAA
Hairpin Sequence:
5'-CCGG-AGTTAACCAAGAAGCACGAAA-CTCGAG-TTTCGTGCTTCTTGGTTAACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTTAACCAAGAAGCACGAAACTCGAGTTTCGTGCTTCTTGGTTAACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTTAACCAAGAAGCACGAAACTCGAGTTTCGTGCTTCTTGGTTAACT-3'

Other clones with same target sequence:

(none)