Construct: shRNA TRCN0000377712

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003661.3-1114s21c1
DNA Barcode:
GAACAGGTGGAGAGGGTTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
APOL1 (8542)
Gene Description:
apolipoprotein L1
Transcript:
RefSeq NM_003661.3 (NON-CURRENT)
Match location:
Position 1114 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000377712)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACAGGTGGAGAGGGTTAAT
Hairpin Sequence:
5'-CCGG-GAACAGGTGGAGAGGGTTAAT-CTCGAG-ATTAACCCTCTCCACCTGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACAGGTGGAGAGGGTTAATCTCGAGATTAACCCTCTCCACCTGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACAGGTGGAGAGGGTTAATCTCGAGATTAACCCTCTCCACCTGTTC-3'

Other clones with same target sequence:

(none)