Construct: shRNA TRCN0000378206

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002376.5-2645s21c1
DNA Barcode:
ACGCCAATAACTGCGACTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MARK3 (4140)
Gene Description:
microtubule affinity regulating kinase 3
Transcript:
RefSeq NM_002376.5 (NON-CURRENT)
Match location:
Position 2645 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000378206)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGCCAATAACTGCGACTATG
Hairpin Sequence:
5'-CCGG-ACGCCAATAACTGCGACTATG-CTCGAG-CATAGTCGCAGTTATTGGCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGCCAATAACTGCGACTATGCTCGAGCATAGTCGCAGTTATTGGCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGCCAATAACTGCGACTATGCTCGAGCATAGTCGCAGTTATTGGCGT-3'

Other clones with same target sequence:

(none)