Construct: shRNA TRCN0000378420

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011861.2-1353s21c1
DNA Barcode:
TTCGTGTACGGGCACTCTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pacsin1 (23969)
Gene Description:
protein kinase C and casein kinase substrate in neurons 1
Transcript:
RefSeq NM_011861.2 (NON-CURRENT)
Match location:
Position 1353 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000378420)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCGTGTACGGGCACTCTATG
Hairpin Sequence:
5'-CCGG-TTCGTGTACGGGCACTCTATG-CTCGAG-CATAGAGTGCCCGTACACGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCGTGTACGGGCACTCTATGCTCGAGCATAGAGTGCCCGTACACGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCGTGTACGGGCACTCTATGCTCGAGCATAGAGTGCCCGTACACGAA-3'

Other clones with same target sequence:

(none)