Construct: shRNA TRCN0000378423

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153587.2-2821s21c1
DNA Barcode:
ATCTGCCTCGTGACGAATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rps6ka5 (73086)
Gene Description:
ribosomal protein S6 kinase, polypeptide 5
Transcript:
RefSeq NM_153587.2 (NON-CURRENT)
Match location:
Position 2821 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000378423)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCTGCCTCGTGACGAATATT
Hairpin Sequence:
5'-CCGG-ATCTGCCTCGTGACGAATATT-CTCGAG-AATATTCGTCACGAGGCAGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCTGCCTCGTGACGAATATTCTCGAGAATATTCGTCACGAGGCAGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCTGCCTCGTGACGAATATTCTCGAGAATATTCGTCACGAGGCAGAT-3'

Other clones with same target sequence:

(none)