Construct: shRNA TRCN0000378456

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172461.3-1411s21c1
DNA Barcode:
GAACTAGATGAACGGACTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nek11 (208583)
Gene Description:
NIMA (never in mitosis gene a)-related expressed kinase 11
Transcript:
RefSeq NM_172461.3 (CURRENT)
Match location:
Position 1411 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000378456)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACTAGATGAACGGACTTTG
Hairpin Sequence:
5'-CCGG-GAACTAGATGAACGGACTTTG-CTCGAG-CAAAGTCCGTTCATCTAGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACTAGATGAACGGACTTTGCTCGAGCAAAGTCCGTTCATCTAGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACTAGATGAACGGACTTTGCTCGAGCAAAGTCCGTTCATCTAGTTC-3'

Other clones with same target sequence:

(none)