Construct: shRNA TRCN0000378457

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028778.3-180s21c1
DNA Barcode:
GGGCTCATCAAGTCGCCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nuak2 (74137)
Gene Description:
NUAK family, SNF1-like kinase, 2
Transcript:
RefSeq NM_028778.3 (NON-CURRENT)
Match location:
Position 180 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000378457)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGCTCATCAAGTCGCCTAAA
Hairpin Sequence:
5'-CCGG-GGGCTCATCAAGTCGCCTAAA-CTCGAG-TTTAGGCGACTTGATGAGCCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGCTCATCAAGTCGCCTAAACTCGAGTTTAGGCGACTTGATGAGCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGCTCATCAAGTCGCCTAAACTCGAGTTTAGGCGACTTGATGAGCCC-3'

Other clones with same target sequence:

(none)