Construct: shRNA TRCN0000378672

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002980.2-1217s21c1
DNA Barcode:
GGAAATGAAGTCAGCCATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCTR (6344)
Gene Description:
secretin receptor
Transcript:
RefSeq NM_002980.2 (CURRENT)
Match location:
Position 1217 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000378672)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAAATGAAGTCAGCCATTAT
Hairpin Sequence:
5'-CCGG-GGAAATGAAGTCAGCCATTAT-CTCGAG-ATAATGGCTGACTTCATTTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAAATGAAGTCAGCCATTATCTCGAGATAATGGCTGACTTCATTTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAAATGAAGTCAGCCATTATCTCGAGATAATGGCTGACTTCATTTCC-3'

Other clones with same target sequence:

(none)