Construct: shRNA TRCN0000378935

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014582.2-143s21c1
DNA Barcode:
TGGTGGTCGATAAGGACTTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OBP2A (29991)
Gene Description:
odorant binding protein 2A
Transcript:
RefSeq NM_014582.2 (NON-CURRENT)
Match location:
Position 143 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000378935)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGTGGTCGATAAGGACTTTC
Hairpin Sequence:
5'-CCGG-TGGTGGTCGATAAGGACTTTC-CTCGAG-GAAAGTCCTTATCGACCACCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGTGGTCGATAAGGACTTTCCTCGAGGAAAGTCCTTATCGACCACCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGTGGTCGATAAGGACTTTCCTCGAGGAAAGTCCTTATCGACCACCA-3'

Other clones with same target sequence:

(none)