Construct: shRNA TRCN0000378985

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004636.2-1702s21c1
DNA Barcode:
CTGTCACCAGCATGCAAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SEMA3B (7869)
Gene Description:
semaphorin 3B
Transcript:
RefSeq NM_004636.2 (NON-CURRENT)
Match location:
Position 1702 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000378985)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTCACCAGCATGCAAATTT
Hairpin Sequence:
5'-CCGG-CTGTCACCAGCATGCAAATTT-CTCGAG-AAATTTGCATGCTGGTGACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTCACCAGCATGCAAATTTCTCGAGAAATTTGCATGCTGGTGACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTCACCAGCATGCAAATTTCTCGAGAAATTTGCATGCTGGTGACAG-3'

Other clones with same target sequence:

(none)